172158-AF
Tyrosinase-Related Protein-1 (5, 6 dihydroxyindole 2 carboxylic acid oxidase, 6-dihydroxyindole-2-carboxylic acid oxidase, Associated with iris pigmentation, CAS2, Catalase B, CATB, DHICA oxidase, GlycoProtein75, GP75, Melanoma antigen gp75, Tyrosinase-related Protein 1, TYRP1, TYRRP, TRP-1) (BSA & Azide Free)
Size
£646.00
- SKU:
- 172158-AF
- Purity:
- Purified by Protein A/G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- SK-MEL-23 cells. Cellular Localization: Cytoplasmic
- Clone:
- SPM456
- Format:
- Supplied as a liquid in PBS. Also available with BSA and azide, see 172158.
- Extra Details:
- It reacts with a 75kD melanocyte-specific gene product, identified as Tyrosinase-related protein-1 (TRP-1). It is involved in melanin synthesis. TRP1 is present on the melanosomal membranes of melanoma, normal melanocytes and nevi. Recent evidence suggests that TRP-1 is involved in maintaining stability of tyrosinase protein and modulating its catalytic activity. TRP-1 is also involved in maintenance of melanosome ultrastructure and affects melanocyte proliferation and cell death. Applications: Suitable for use in Immunofluorescence, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/million cells Immunofluorescence: 1-2ug/ml Immunohistochemistry (Frozen only): 0.5-1ug/ml for 30 minutes at RT Optimal dilution for a specific application should be determined. Positive Control: SK-MEL-23, SK-MEL-19, SK-MEL-30, SK-MEL-37 cells or Melanoma.. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice