TGF-alpha (EGF-like TGF, ETGF, TFGA, TGF Type 1, TGFA, Wa1, Waved 1)

Size

£422.00

SKU:: 172154
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to aa34-43 from human TGFalpha. Cellular Localization: Cytoplasmic and Extracellular (secreted)
Clone:: SPM542
Format:: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 172154-AF.
Extra Details:: This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this MAb is completely blocked by the peptide used for raising this antibody. TGFa (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 0.5-1ug/million cells Immunofluorescence: 1-2ug/ml Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher. Positive Control: Jurkat or Ramos cells, Heart, Kidney, Pituitary, Breast cancer, Melanoma Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice