172154-AF-ML650
TGF-alpha (EGF-like TGF, ETGF, TFGA, TGF Type 1, TGFA, Wa1, Waved 1) (BSA & Azide Free) (MaxLight 650)
Size
£876.00
- SKU:
- 172154-AF-ML650
- Purity:
- Purified by Protein A/G affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to aa34-43 from human TGFalpha. Cellular Localization: Cytoplasmic and secreted
- Clone:
- SPM542
- Format:
- Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™650.
- Extra Details:
- MaxLight™650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000. This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this MAb is completely blocked by the peptide used for raising this antibody. TGFa (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, FLISA, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 0.5-1ug/10e6 cells Immunofluorescence: 1-2ug/ml FLISA: Coating antibody Immunoprecipitation: 1-2ug/500ug protein Immunohistochemistry (Frozen/paraffin): 1-2ug/ml for 30 minutes at RT. Staining of paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher. Positive Control: Jurkat or Ramos cells, heart, kidney, pituitary, Breast cancer, melanoma Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice