Retinol Binding Protein (Cellular Retinol-binding Protein I, CRBP1, CRBP2, RBP1, RBP2, RBP4, RBPC, Retinol binding Protein 1, Retinol binding Protein 1 Cellular, Retinol binding Protein 2 Cellular, Retinol binding Protein 4 plasma, RBP)

Size

£422.00

SKU:: 172148
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Retinol binding protein purified from human plasma. Cellular Localization: Cytoplasmic
Clone:: SPM442
Format:: Supplied as a liquid in 10mM PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 172148-AF.
Extra Details:: Recognizes a protein of 21kD-25kD, identified as retinol binding protein (RBP). Its epitope localizes between aa 74-182 of human RBP. This MAb recognizes reduced and carboxy-methylated RBP (RCM-RBP) as well as the circulatory RBP but not the native RBP, thereby suggesting that its epitope becomes accessible either on unfolding or upon binding of RBP to transthyretin (prealbumin). RBP is responsible for distributing retinol from the retinoid stores in the liver to the various target tissues. Once secreted into the blood with bound retinol, the vitamin carrier circulates complexed with transthyretin prior to vitamin delivery at the plasma membrane through a receptor-mediated mechanism. Applications: Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 1-2ug/ml Immunoprecipitation: 1-2ug/500ug protein Western Blot: 0.5-1ug/ml Immunohistochemistry (Frozen only): 1-2ug/ml for 30 min at RT Optimal dilution for a specific application should be determined. Positive Control: Liver or placenta Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice