172145-AF-FITC

pS2 (BCEI, Breast Cancer Estrogen Inducible Protein, Gastrointestinal Trefoil Protein, Gastrointestinal trefoil Protein pS2, HP1A, HPS2, pNR2, pNR-2, TFF1, Trefoil Factor 1) (FITC)

Size

£876.00

SKU:: 172145-AF-FITC
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide of 28 amino acid residues corresponding to CFDDTVRGVPWCFYPNTIDVPPEEECEF (aa57-84) from the C-terminus of human pS2. Cellular Localization: Cytoplasmic
Clone:: SPM573
Format:: Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Fluorescein isothiocyanate (FITC).
Extra Details:: It recognizes a polypeptide of 6.5kD, identified as pS2 estrogen-regulated protein. Its epitope is localized between aa57-84 of human pS2 protein. pS2 is a trefoil peptide. Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. These peptides contain three intrachain disulfide bonds, forming the trefoil motif, or P-domain. pS2 is known to form dimers and this dimerization is thought to play a role in its protective and healing properties. About 60% of breast carcinomas are positive for pS2. Staining is cytoplasmic, often with localization to the Golgi apparatus. pS2 is shown to be localized in normal stomach mucosa, gastric fluid, goblet cells in the colon and small intestine, and in ulcerations of the gastrointestinal tract. Several studies have shown that pS2 is primarily expressed in estrogen receptor-positive breast tumors and it may define a subset of estrogen-dependent tumors that displays an increased likelihood of response to endocrine therapy. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, FLISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/million cells Immunofluorescence: 0.5-1ug/ml Western Blot: 0.5-1.0ug/ml Immunoprecipitation: 0.5-1µg/500ug protein lysate Immunohistochemistry (Frozen & Formalin-fixed): 0.5-1.0ug/ml for 30 minutes at RT [Staining of formalin/paraffin tissues REQUIRES digestion with Pronase E (Protease XXV from Sigma; Cat# P 6911) at 1mg/ml PBS, 15 min at 37°C] (Note that Heat Induced Epitope Retrieval is not suitable for this antibody) Optimal dilution for a specific application should be determined. Positive Control: Spent medium of MCF-7 cells, treated with estrogen. Normal stomach. About 60% of breast carcinomas are positive for pS2, especially those that are also positive for estrogen and/or progesterone receptor. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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