Progesterone Receptor (NR3C3, Nuclear receptor subfamily 3 group C member 3, PGR, PR, PRA, PRB, Progesterone receptor form A, Progesterone receptor form B)

Size

£422.00

SKU:: 172142
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant human Progesterone Receptor protein. Cellular Localization: Nuclear
Clone:: SPM566
Format:: Supplied as a liquid in 10mM PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 172142-AF.
Extra Details:: This MAb is specific to progesterone receptor and shows minimal cross-reaction with other members of the family. Progesterone receptor is expressed as two major isoforms, PR-A (81kD) and PR-B (116kD). Expression of PgR has been suggested to reflect a intact estrogen regulatory machinery and therefore, predict better clinical response to endocrine therapy than ER alone. It is excellent for immunohistochemical staining of formalin/paraffin tissues. Applications: Suitable for use in Western Blot, Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml for 2 hours at RT Immunohistochemistry (Frozen & Formalin-fixed): 0.5-1ug/ml for 30 min at RT (IHC recommended) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined. Positive Control: T47-D Cells or Breast Cancers. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice