172142-AF-APC
Progesterone Receptor (NR3C3, Nuclear receptor subfamily 3 group C member 3, PGR, PR, PRA, PRB, Progesterone receptor form A, Progesterone receptor form B) (APC)
Size
£876.00
- SKU:
- 172142-AF-APC
- Purity:
- Purified by Protein A/G affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant human Progesterone Receptor protein. Cellular Localization: Nuclear
- Clone:
- SPM566
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
- Extra Details:
- This MAb is specific to progesterone receptor and shows minimal cross-reaction with other members of the family. Progesterone receptor is expressed as two major isoforms, PR-A (81kD) and PR-B (116kD). Expression of PgR has been suggested to reflect a intact estrogen regulatory machinery and therefore, predict better clinical response to endocrine therapy than ER alone. It is excellent for immunohistochemical staining of formalin/paraffin tissues. Applications: Suitable for use in Western Blot, Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml for 2 hours at RT Immunohistochemistry (Frozen & Formalin-fixed): 0.5-1ug/ml for 30 min at RT (IHC recommended) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined. Positive Control: T47-D Cells or Breast Cancers. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. APC is light sensitive. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice