172132-AF

Neurofilament (NEFH, Neurofilament H, Neurofilament Heavy Polypeptide 200kD, Neurofilament Triplet H Protein, NF-H, NF200, NF-H) (BSA & Azide Free)

Size

£646.00

SKU:: 172132-AF
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human recombinant neurofilament protein. Cellular Localization: Cytoplasm, cytoskeleton, cell projection and axon
Clone:: SPM563
Format:: Supplied as a liquid in PBS. Also available with BSA and azide, see 172132.
Extra Details:: This MAb reacts with a 200kD protein, identified as heavy sub-unit of neurofilaments (NF-H). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kD (NF-L), 160kD (NF-M) and 200kD (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament. Applications: Suitable for use in Flow Cytometry, ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1-2ug/million cells Western Blot: 2-4ug/ml Immunohistochemistry (Frozen & Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 minutes at 95ºC followed by cooling at RT for 20 minutes. Optimal dilutions to be determined by the researcher. Positive Control: Brain, Neuroblastoma Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice