MUC5AC (Apomucin Major Airway GlycoProtein, Mucin 5 subtype AC tracheobronchial, Mucin 5 Subtypes A And C, Mucin 5AC oligomeric mucus/gel forming, Tracheobronchial Mucin, TBM, Mucin 5AC, Gastric Mucin)

Size

£422.00

SKU:: 172129
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient. Cellular Localization: Cytoplasmic
Clone:: SPM297
Format:: Supplied as a liquid in 10mM PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 172129-AF.
Extra Details:: This MAb recognizes the peptide core of gastric mucin M1 (>1,000kD) (recently identified as Mucin 5AC). Its epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. Antibody to gastric mucin M1 reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1-2ug/million cells Immunofluorescence: 1-2ug/ml Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris, 1mM EDTA, pH 9.0, for 45 minutes followed by cooling at RT for 20 minutes) Optimal dilutions to be determined by the researcher. Positive Control: Human, adult normal and gastric carcinoma Human, adult colon carcinoma Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice