MAGE-1 (MZ2 E, cancer/testis antigen 1.1, CT1.1, MAGE1A, MAGEA1, Melanoma antigen family A 1, Melanoma Associated antigen 1, Melanoma Associated antigen MZ2 E)

Size

£422.00

SKU:: 172121
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human MAGE-A1 full length recombinant protein. Cellular Localization: Cytoplasmic
Clone:: SPM282
Format:: Supplied as a liquid in 10mM PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 172121-AF.
Extra Details:: Recognizes a protein of 42-46kD, identified as MAGE-1. This MAb does not cross-react with MAGE-2, -3, -4, -6 -9, -10, -or -12 protein. Human malignant neoplasms carry rejection antigens that are recognized by the patients' autologous, tumor directed and specific, cytolytic, CD8+ T lymphocyte clones (CTL). The MAGE family of genes codes an important group of antigens. It was identified that melanomas and primary glial brain tumors express common melanoma associated antigens (MAAs). Because MAGE-1 is expressed on a significant proportion of human neoplasms of various histological types (melanoma, brain tumors of glial origin, neuroblastoma, non-small cell lung cancer, breast, gastric, colorectal, ovarian, renal cell carcinomas) and not on normal tissues, the encoded antigen may serve as a marker of early detection and target for cancer immunotherapy. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/million cells Immunofluorescence: 1-2ug/ml Western Blot: 0.5-1ug/ml Immunoprecipitation: 1-2µg/500ug protein lysate Immunohistochemistry (Frozen & Formalin-fixed): 0.5-1ug/ml for 30 minutes at RT (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined. Positive Control: Melanoma cell lines. Melanomas, gliomas, neuroblastoma, non-small cell lung cancer, breast, gastric, colorectal, ovarian, and renal cell carcinomas. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice