172115-AF
Involucrin (Inv, Invo, IVL) (BSA & Azide Free)
Size
£646.00
- SKU:
- 172115-AF
- Purity:
- Purified by Protein A/G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Purified involucrin from human keratinocytes. Cellular Localization: Cytoplasmic
- Clone:
- SPM259
- Format:
- Supplied as a liquid in PBS. Also available with BSA and azide, see 172115.
- Extra Details:
- Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/million cells Immunofluorescence: 1-2ug/ml Immunoprecipitation: 1-2ug/500ug protein Western Blot: 0.5-1ug/ml Immunohistochemistry (Frozen & Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues is enhanced by digestion with trypsin at 1mg/ml PBS for 5 min at 37°C) Optimal dilution for a specific application should be determined. Positive Control: MCF-7 cells. Localized to upper spinous and granular layers in normal skin. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice