172114-AF-APC

Insulin-like Growth Factor-1 (Insulin-like Growth Factor IA, IGF-IA, Insulin-like Growth Factor IB, IGF-IB, Mechano Growth Factor, MGF, Somatomedin-C, IGF-1) (APC)

Size

£876.00

SKU:: 172114-AF-APC
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified human IGF-1 protein. Cellular Localization: Cytoplasmic (Secreted)
Clone:: SPM406
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Extra Details:: This antibody is specific to Insulin-like Growth Factor (IGF-1) and shows minimal cross-reaction with IGF-11, Proinsulin, MSF, and Insulin. IGF-1 is a polypeptide growth factor with two isoforms that are produced by alternative splicing. Isoform 1 is also known as IGF-IB while isoform 2 is known as IGF-IA. IGF-1 stimulates the proliferation of a wide range of cell types including muscle, bone and cartilage tissue. It functions as an autocrine regulator of growth. Activation of IGF system has emerged as a key factor for tumor progression and resistance to apoptosis in many cancers like those of breast, thyroid and colon. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, FLISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/million cells Immunofluorescence: 1-2ug/ml Immunoprecipitation: 1-2ug/500ug protein Western Blot: 0.5-1.0ug/ml Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT In-vitro Neutralization of Biological Activity of IGF-1 (Order Ab without Azide) Optimal dilution for a specific application should be determined. Positive Control: Pancreas or brain. Breast, Thyroid or Colon Cancers; IGF-1 recombinant protein Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. APC is light sensitive. Note: Applications are based on unconjugated antibody.

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