172108-AF-HRP

HSP27 (Heat Shock Protein 27, Heat shock 27kD Protein, Estrogen-regulated 24kD Protein, Heat shock 25kD Protein 1, Heat shock 28kD Protein 1, Heat shock Protein beta-1, HMN2B, Hsp25, Hsp28, HspB1, Stress-responsive Protein 27, SRP27) (HRP)

Size

£876.00

SKU:: 172108-AF-HRP
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Partially purified hsp27 (earlier called 24K) protein from breast cancer MCF-7 cells. Cellular Localization: Predominantly cytoplasmic with some nuclear
Clone:: SPM252
Format:: Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with horseradish peroxidase (HRP).
Extra Details:: It recognizes a 24-27kD estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced “p29” and “24K” protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.25-0.5ug/ml Immunoprecipitation: 0.5-1µg/500ug protein lysate Immunohistochemistry (Frozen & Formalin-fixed): 0.5-1ug/ml for 30 minutes at RT (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined. Positive Control: ~50% of Breast Carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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