172106-AF-BIOTIN

Hepatocyte Specific Antigen (Biotin)

Size

£876.00

SKU:: 172106-AF-BIOTIN
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Extract of a formalin-fixed, rejected-allograft of a human liver. Cellular Localization: Finely granular cytoplasmic
Clone:: SPM582
Format:: Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Biotin.
Extra Details:: Hepatocyte Specific Antigen, also called Hepatocyte Paraffin 1 or Hep Par 1, localizes to the mitochondria of hepatocytes. It is a sensitive marker for distinguishing hepatocellular carcinomas (HCC) from other metastatic carcinomas as well as cholangio-carcinomas. HCC’s occur primarily in the stomach, but they are also found in many other organs. The Hepatocyte Specific Antigen may also be a useful marker for intestinal metaplasia. Reportedly, strong expression of the Hepatocyte Specific Antigen correlates with smaller tumor size and longer patient survival. Occasionally, Hepatocyte Specific Antigen is also found in gastric carcinomas as well as in a few other non-hepatic tumors. Applications: Suitable for use in Immunofluorescence, Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 0.5-1.0ug/ml Immunohistochemistry (Frozen & Formalin-fixed): 0.5-1.0ug/ml for 30 minutes at RT (Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined. Positive Control: Liver or Hepatocellular Carcinoma (HCC) Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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