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172103-AF

Granulocyte Marker (Pluripoietin, Filgrastim, Lenograstim, granulocyte colony-stimulating factor, G-CSF) (BSA & Azide Free)

Size

£646.00

SKU:
172103-AF
Purity:
Purified by Protein A/G affinity chromatography.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Mouse
Immunogen:
Nuclei from pokeweed mitogen stimulated human peripheral blood lymphocytes. Cellular Localization: Cytoplasmic
Clone:
SPM250
Format:
Supplied as a liquid in PBS. Also available with BSA and azide, see 172103.
Extra Details:
Recognizes an unidentified antigen in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell type in human tissues. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. MAb BM-2 reacts with early precursor and mature forms of human myeloid cells. This MAb is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 0.5-1ug/million cells Immunofluorescence: 1-2ug/ml Immunohistochemistry (Frozen & Formalin-fixed): 0.5-1ug/ml for 30 minutes at RT. Optimal dilutions to be determined by the researcher. Positive Control: Tonsil or lymph node Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Conditions:
Blue Ice