172089

Ep-CAM (CD326, Adenocarcinoma-Associated Antigen, Cell Surface GlycoProtein Trop-1, EGP2, EGP314, EGP40, Epithelial Cell Adhesion Molecule, Epithelial GlycoProtein 314, ESA, KSA, TACD1, TACSTD1, TROP1, Tumor-Associated Calcium Signal Transducer 1, ECS-1, Epidermal Surface Antigen 1, ESA1, FLOT2, Flotillin-2, M17S1, Membrane Component, Chromosome 17, Surface Marker-1, REG-1, Reggie-1, Reggie-2)

Size

£448.00

SKU:: 172089
Purity:: Supernatant
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Rat small intestine cells. Cellular Localization: Cell surface
Clone:: SPM534
Format:: Supplied as a liquid in 0.05% sodium azide.
Extra Details:: Epitope of SPM534 and SPM535 monoclonal antibodies are different. EGP40 is a 40-43kD transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5-10ul/million cells Immunoprecipitation: 5-10ul/million cells Immunofluorescence: 1:50-1:100 Western Blot: 1:100-1:200 Immunoprecipitation: 5-10ul/500ug protein lysate Immunohistochemistry (Frozen & Formalin-fixed): 1:100-1:200 for 30 min at RT (Formalin/paraffin tissues REQUIRE digestion of tissue sections with pepsin at 1mg/ml Tris-HCl, pH 2.0 for 15 min at RT or 10 min at 37C) Optimal dilution for a specific application should be determined. Positive Control: Rat small intestine or colon Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice