172077-AF-FITC

Cytokeratin 19 (k19, k1cs, Keratin 19 Keratin Type i 40kD, krt19) (FITC)

Size

£876.00

SKU:: 172077-AF-FITC
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human breast cancer MCF-7 cells. Cellular Localization: Cytoplasmic
Clone:: SPM266
Format:: Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Fluorescein isothiocyanate (FITC).
Extra Details:: This MAb reacts with the rod domain of human cytokeratin-19 (CK19), a polypeptide of 40kD. Its epitope maps between amino acid 312-335. CK19 is expressed in sweat gland, mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, and ectocervical epithelium. Anti-CK19 reacts with a wide variety of epithelial malignancies including adenocarcinomas of the colon, stomach, pancreas, biliary tract, liver, and breast. Perhaps the most useful application is the identification of thyroid carcinoma of the papillary type, although 50%-60% of follicular carcinomas are also labeled. Anti-CK19 is a useful marker for detection of tumor cells in lymph nodes, peripheral blood, bone marrow and breast cancer. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, FLISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/million cells Immunofluorescence: 1-2ug/ml Immunoprecipitation: 1-2ug/500ug protein Western Blot: 0.5-1ug/ml Immunohistochemistry (Frozen & Formalin-fixed): 0.5-1ug/ml for 30 min at RT (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined. Positive Control: MCF-7, HeLa, Hep-G2 cells, Breast Cancer Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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