172036-AF
Bromodeoxyuridine (Bromodeoxyuridine, BUdr, BrdU) (BSA & Azide Free)
Size
£646.00
- SKU:
- 172036-AF
- Purity:
- Purified by Protein A/G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Bromodeoxyuridine (BrdU) conjugated to KLH. Cellular Localization: Nuclear
- Clone:
- SPM166
- Format:
- Supplied as a liquid in PBS. Also available with BSA and azide, see 172036.
- Extra Details:
- It reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/million cells Immunoprecipitation: 0.5-1ug/million cells Immunofluorescence: 0.5-1ug/ml Immunohistochemistry (Frozen & Formalin-fixed): 0.5-1ug/ml for 30 minutes at RT (Use Ab at 1ug/ml for 30 minute at RT [For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37oC (Cat. #AP-9008)] Optimal dilution for a specific application should be determined. Positive Control: Cells grown in presence of BrdU or tissues from experimental animals injected with BrdU Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice