172034-AF-BIOTIN

BCL10 (B Cell CLL/lymphoma 10, B Cell Leukemia/lymphoma 10, CARD-containing Molecule enhancing NF-kappa-B, CARD-like apoptotic Protein, caspase-recruiting domain-containing Protein, CED-3/ICH-1 Prodomain Homologous E10-like Regulator, Cellular Homolog of vCARMEN, Cellular-E10, cE10, CIPER, hCLAP, mE10, R-RCD1) (Biotin)

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£876.00

SKU:: 172034-AF-BIOTIN
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human BCL10 recombinant protein (epitope aa122-168). Cellular Localization: Nuclear and cytoplasmic
Clone:: SPM520
Format:: Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with Biotin.
Extra Details:: BCL10, with an N-terminal caspase recruitment domain (CARD), is found in a number of apoptotic regulatory molecules. It was identified through its direct involvement in t(1;14) of mucosa-associated lymphoid tissue (MALT) lymphoma. Expression of BCL10 was shown to induce NFκB activation in a NIK-dependent pathway. This MAb labels subpopulations of normal B and T cells and is a useful tool for the sub-classification of lymphomas. In MALT lymphomas with the t(1;14) translocation, while 55% of MALT lymphomas lacking this translocation exhibited the same labeling pattern, although at a much lower level. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/million cells Immunofluorescence: 1-2ug/ml Western Blot: 0.5-1ug/ml Immunoprecipitation: 1-2g/500ug protein lysate Immunohistochemistry (Frozen & Formalin-fixed): 0.5-1ug/ml for 30 minutes at RT (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined. Positive Control: WEHI-231 or Ramos cells or lymphoma. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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