172030-AF-HRP

Bax (Apoptosis Regulator BAX Cytoplasmic Isoform beta, Apoptosis Regulator BAX Membrane isoform alpha, Bax isoform psi, Bax Protein cytoplasmic isoform delta, Bax Protein Cytoplasmic isoform gamma, Bax zeta, Bcl-2-like Protein 4, BCL2 Associated X Protein, BCL2 Associated X Protein omega, BCL2 Associated X Protein Transcript Variant delta2, BCL2L4) (HRP)

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£876.00

SKU:: 172030-AF-HRP
Purity:: Purified by Protein A/G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: A synthetic peptide, aa3-16 (Cys-GSGEQPRGGGPTSS) of human bax protein. Cellular Localization: Cytoplasmic
Clone:: SPM336
Format:: Supplied as a liquid in 10mM PBS. No preservative added. BSA-Free. Labeled with horseradish peroxidase (HRP).
Extra Details:: Recognizes a protein of 21kD, identified as the Bax protein. This MAb is highly specific to Bax and shows no cross-reaction with Bcl-2a or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5-1ug/ml Immunoprecipitation: 1-2ug/500ug protein lysate Immunohistochemistry (Frozen & Formalin-fixed): 0.5-1ug/ml for 30 minutes at RT (Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined. Positive Control: Jurkat, K562, HL-60, or HeLa Cells. Reed-Sternberg cells in Hodgkin’s lymphomas. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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