171999

TAK1-TAB1, Recombinant, Human, GST-Tag

Size

£918.00

SKU:: 171999
Molecular Weight:: 74
Purity:: Highly Purified (~93%)
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.
Extra Details:: Recombinant human proteins TAK1 (1-303), with N-terminal GST-tag, and TAB1 (437-end), with N-terminal GST-tag,, linked by a small peptide (DFGGGGG), with N-terminal GST-tag,, were expressed by Sf9 insect cells via a baculovirus expression system.using an N-terminal GST tag. Source: Recombinant protein corresponding to human TAK1-TAB1 at N-terminal, fused to GST-tag expressed in Sf9 insect cells via a baculovirus expression system. Molecular Weight: ~74kD Assay Conditions: Assay was performed in a kinase buffer containing 0.2mM DTT using MBP substrate (0.1mg/ml) and 100mM ATP. Reaction was done at 30oC for 35 min. Application: Useful for studying enzyme kinetics, screening inhibitors, and selectivity profiling. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.