171978

PI3 kinase (p110a/p85a), Active, Recombinant, Human

Size

£957.00

SKU:: 171978
Molecular Weight:: 1511108438
Purity:: ~38%
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 45mM Tris-HCl, pH 8.0, 124mM NaCl, 2.4mM KCl, 18mM glutathione, 10% glycerol, 3mM DTT.
Extra Details:: Complex of N-terminal GST-tagged recombinant full-length human p110α (GenBank Accession No. U79143), and recombinant full length, human p85a (no tag) (GenBank Accession No. XM_043865). Coexpressed in a Baculovirus infected Sf9 cell expression system. p110a MW=151kD, p85a MW=84kD. Source: Full length recombinant protein corresponding to active human expressed in Baculovirus infected Sf9. Molecular Weight: ~151, ~84kD Specific Activity: 756 pmol/min/ug A 25ul kinase reaction is conducted in a buffer containing 40mM Tris-HCl (pH 7.4), 20mM MgCl2, 100uM ATP, 200uM PI:PS substrate and enzyme for 15 min at 30°C. ATP reduction is detected. Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.