171977

PHGDH, Recombinant, Human, aa1-533 (Phosphoglycerate Dehydrogenase, PDG)

Size

£957.00

SKU:: 171977
Molecular Weight:: 57.5
Purity:: ~80%
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2mM KCl, 170mM imidazole, 0.04% Tween 20, 20% glycerol.
Extra Details:: Human phosphoglycerate dehydrogenase (PGDH), also known as PDG, amino-acids 1 – 533 (end) (GenBank Accession No. NM_006623) with C-terminal His tag, MW = 57.5kD, expressed in an E. coli expression system. Source: Recombinant protein corresponding to aa1-533 from human PHGDH at C-terminal, fused to His-tag expressed in E.coli. Molecular Weight: ~57.5kD Specific Activity: 80 pmol/min/ug Assay Conditions: The reaction mixture contained 100mM Tris-HCl, pH 8.8, 400mM NaCl, 0.2mM DTT, 2.5mM 3-phospho-D-glyceric acid (Sigma P8877), 1mM NAD, and various amount of PHGDH in a final volume of 50ul. Reactions were initiated with the addition of PHGDH, and the increase in NADH fluorescence (Ex340/Em460 nm) was continuously monitored for 10 min at room temperature. Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap