171944

PADI-4, Recombinant, Human (Peptidyl arginine deiminase, type IV)

Size

£849.00

SKU:: 171944
Molecular Weight:: 75
Purity:: Purified (~65%)
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 3mM DTT, 80ug/ml FLAG peptide, 20% glycerol.
Extra Details:: Human PADI-4, also known as Peptidyl arginine deiminase, type IV, (GenBank Accession No. NM_012387), 1-663(end), with C-terminal FLAG-tag, MW= 75kD, expressed in a Baculovirus infected Sf9 cell expression system. Source: Recombinant protein corresponding to aa1-663 from human PADI-4 at C-terminal, fused to FLAG-tag expressed in Baculovirus infected Sf9. Molecular Weight: ~75kD Specific Activity: 80 pmole/min/ug Assay Conditions: Enzyme reaction was done in a buffer (50mM Tris, pH 7.4, 50mM NaCl, 10mM CaCl2, and 2mM DTT) containing PADI4 and 10mM BAEE as a substrate for 25 min at 37°C. Amount of ammonia produced was measured and used for specific activity calculation. Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.