171938

Nucleosome, Recombinant, Human (Biotin)

Size

£1,150.00

SKU:: 171938
Molecular Weight:: 113.8
Purity:: Highly Purified (~99%)
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 1mM DTT, 20% glycerol. Labeled with Biotin.
Extra Details:: Human recombinant nucleosomes consisting of 147 bp DNA (PCR) and 2 molecules each of histones H2A (a.a.2-130(end)), H2B (a.a.2-126(end)), H3 (a.a.2-137(end)), and H4 (a.a.2-103(end)), expressed in an E. coli expression system. GenBank Accession Nos. are NM_033445, NM_003528, NM_003532, and NM_003548, respectively. Each histone protein has an N-terminal His-tag each H2B has a C-terminal Cys added as a biotinylation site.Total histone MW (before biotinylation) =113.8kD. Source: Recombinant protein corresponding to aa2-137 of Nucleosome at the N-terminus, expressed in E.coli. Molecular Weight: ~113.8kD Application: Substrate for histone methyltransferase assays. Ideal for screening small molecular inhibitors of histone methyltransferases for drug discovery and HTS applications. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap