171937

Nucleosome, Recombinant, Human

Size

£1,053.00

SKU:: 171937
Molecular Weight:: 113.8
Purity:: ~99%
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 1mM EDTA, 2mM DTT, 20% glycerol.
Extra Details:: Human recombinant nucleosomes consisting of 147 bp DNA (PCR) and 2 molecules each of histones H2A (a.a.2-130(end)), H2B (a.a.2-126(end)), H3 (a.a.2-137(end)), and H4 (a.a.2-103(end)), expressed in an E. coli expression system. Each histone protein has an N-terminal His-tag. Total histone MW=113.8kD. Source: Recombinant protein corresponding to aa2-137 from histone proteins at the N-Terminus, expressed in E.coli. Molecular Weight: ~113.8kD Application: Substrate for histone methyltransferase assays. Ideal for screening small molecular inhibitors of histone methyltransferases for drug discovery and HTS applications. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -80°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.