171930

NEK9, Active, Recombinant, Human (NERCC)

Size

£907.00

SKU:: 171930
Molecular Weight:: 92
Purity:: Purified (~38%)
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 40mM Tris-HCl, pH 8, 110mM sodium chloride, 2.2mM potassium chloride, 4mM Glutathione, 3mM DTT, 20% glycerol.
Extra Details:: Human NEK9, also known as NERCC (GenBank Accession No. NM_033116), a.a1-979 (end), delta 346-731, with N-terminal GST tag, MW =92kD, expressed in Sf9 insect cells via a baculovirus expression system Source: Recombinant protein corresponding to human NEK9 at N-terminal, fused to GST-tag expressed in Sf9 insect cells via a baculovirus expression system. Molecular Weight: ~92kD Specific Activity: 147pmol/min/ug Assay Buffer: Enzyme reaction is conducted in a Kinase Buffer (50mM Tris pH 7.4, 20mM MgCl2, 0.1mg/ml BSA and 1mM DTT) containing 20 CM ATP and 0.2mg/ml MBP for 30 minutes at 30oC. Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.