171907

IDH2, Recombinant, Human (isocitrate dehydrogenase 2)

Size

£797.00

SKU:: 171907
Molecular Weight:: 52
Purity:: ≥95%
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 45mM Tris-HCl, pH 8.0, 124mM sodium chloride, 2.4mM potassium chloride , 100ug/ml FLAG peptide, 3mM DTT, 10% glycerol.
Extra Details:: Recombinant protein corresponding to aa1-452 from human IDH2 at C-terminal, fused to Flag-tag expressed in baculovirus infected Sf9 cell expression system. Uniprot/Accession: NM_002168 Molecular Weight: ~52kD Assay Conditions: The reaction mixture contained 50mM Tris, pH 7.4, 10mM MgCl2, 200uM DL-isocitrate, 200uM NADP+, and various amount of IDH2 in a volume of 200ul. Read abs340 using Tecan Infinite 1000 (5 flash/10ms settle time). Molar extinction coefficient of NADPH is 6220M-1cm-1. Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Amino Acid Sequence: MAGYLRVVRSLCRASGSRPAWAPAALTAPTSQEQPRRHYADKRIKVAKPVVEMDGDE MTRIIWQFIKEKLILPHVDIQLKYFDLGLPNRDQTDDQVTIDSALATQKYSVAVKCATITP DEARVEEFKLKKMWKSPNGTIRNILGGTVFREPIICKNIPRLVPGWTKPITIGRHAHGDQ YKATDFVADRAGTFKMVFTPKDGSGVKEWEVYNFPAGGVGMGMYNTDESISGFAHSC FQYAIQKKWPLYMSTKNTILKAYDGRFKDIFQEIFDKHYKTDFDKNKIWYEHRLIDDMVA QVLKSSGGFVWACKNYDGDVQSDILAQGFGSLGLMTSVLVCPDGKTIEAEAAHGTVTR HYREHQKGRPTSTNPIASIFAWTRGLEHRGKLDGNQDLIRFAQMLEKVCVETVESGAM TKDLAGCIHGLSNVKLNEHFLNTTDFLDTIKSNLDRALGRQDYKDDDDK Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Dry Ice