Histone H3, Recombinant, Human (H4 Tetramer)

Size

£977.00

SKU:: 171899
Molecular Weight:: 57
Purity:: ~80%
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 45mM Tris pH 7.5, 2M NaCl, 1mM EDTA, 1mM DTT, 10% glycerol.
Extra Details:: Human recombinant his-tone octamer consisting of 2 molecules each of N-terminal His-tagged histones H2A (a.a.2-130(end)), H2B (a.a.2-126(end)), H3 (a.a.2-137(end)), and H4 (a.a.2-103(end)), expressed in E. coli. GenBank Accession Nos. are NM_033445, NM_003528, NM_003532, and NM_003548, respectively. H2B has been biotin-labeled via thiol modification of an additional C-terminal cysteine prior to assembly. Global MW = 117kD. Source: Full length recombinant protein corresponding to aa1-136 of Histone H3/H4 Tetramer at the N-terminus, fused to His-tag expressed in E.coli. Molecular Weight: ~57kD Application: A perfect substrate for histone methyltransferases. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.