FYN A, Active, Recombinant, Human

Size

£925.00

SKU:: 171864
Molecular Weight:: 61
Purity:: Purified (~51%)
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 45mM Tris-HCl, pH 8.0, 124mM sodium chloride, 2.4mM potassium chloride, 18mM glutathione, 10% glycerol.
Extra Details:: Human FYN A (GenBank Accession No. NM_002037), full length with N-terminal Flag tag, a.a 2-537(end), MW= 61kD, expressed in Sf9 insect cells. Source: Full length recombinant protein corresponding to aa2-537 from human FYN A at N-terminal, fused to Flag-tag expressed in Sf9 insect cells. Molecular Weight: ~61kD Specific Activity: 200mole/min/ug. Assay Conditions: Enzyme reaction is conducted in a Kinase Buffer (50mM Tris pH 7.4, 20mM MgCl2, 0.1mg/ml BSA, 1mM DTT) containing 20uM ATP and 0.2mg/ml poly-(Glu-Tyr) for 30 min at 30°C. Enzyme activity is measured using Kinase-Glo plus reagents (Promega). Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap