FGFR4, Active, Recombinant, Human

Size

£907.00

SKU:: 171860
Molecular Weight:: 65
Purity:: ~79% (SDS-PAGE)
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM sodium chloride, 2.2mM potassium chloride, 4mM Glutathione, 3mM DTT, 20% glycerol.
Extra Details:: Human FGFR4, (GenBank Accession No. NM_002011) with N-terminal GST tag, MW= 65kD, expressed in Sf9 cells via a baculovirus expression system Source: Recombinant protein corresponding to human FGFR4 at N-terminal, fused to GST tag expressed in Sf9 cells. Molecular Weight: ~65kD Specific Activity: 50pmole/min/ug Assay Conditions: Reaction is conducted in a Kinase Buffer (50mM Tris pH 7.4, 20mM MgCl2, 0.1mg/ml BSA and 1mM DTT) containing 20uM ATP and 0.2mg/ml poly-(Glu-Tyr) for 30 min at 30°C. Enzyme activity is measured using Kinase-Glo plus reagents (Promega). Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.