CDK5/p25, Recombinant, Human, GST-Tag

Size

£912.00

SKU:: 171849
Molecular Weight:: 60
Purity:: ~94%
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2mM KCl, 4mM glutathione, 0.04% Tween 20, 20% glycerol.
Extra Details:: Complex of N-terminal GST-tagged recombinant full length human CDK5 (aa1-292 (end)) (NM_004935), and N-terminal GST-tagged p25 (aa99-307 (end)) (NM_003885), co-expressed in a Baculovirus-infected Sf9 cell expression system. Molecular Weight: ~60kD (CDK5), 50kD (p25) Specific Activity: 312pmol/min/ug Assay Conditions: Assay was done in a Kinase buffer using Histone H1 as a substrate with 20uM ATP at 30°C for 30min. Application: Useful for three dimensional structure studies. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.