CDK5, Active Human Recombinant Protein (p35)

Size

£912.00

SKU:: 171848
Molecular Weight:: 5949
Purity:: ~60%
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2mM KCl, 16mM glutathione, 3mM DTT, 20% glycerol.
Extra Details:: Recombinant full-length human CDK5 (59kD) and human p35 (60kD), both with N-terminal GST tags, were co-expressed in Sf9 cells using a baculovirus expression system. The CDK5 gene accession number is NM_004935 and p35 gene accession number is NM_003885. Source: Full length recombinant protein corresponding to human CDK5 at N-terminal, fused to GST tag expressed in Sf9 cells using a baculovirus expression system. Molecular Weight: ~59kD, 49kD Specific Activity: 180pmole/min/ug Assay conditions: Assay was done in Kinase buffer containing 0.2mM DTT using Histone 1 substrate (0.2mg/ml) and 20mM ATP. Reaction was done at 30°C for 45 min. Amount of ATP transferred. Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.