CDK2, Active, Recombinant, Human (CyclinA2)

Size

£912.00

SKU:: 171847
Molecular Weight:: 3457
Purity:: ≥95%
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 20mM NaH2PO4/Na2HPO4, pH 8.0, 400mM sodium chloride, 20mM Glutathione, 2mM DTT, 10% glycerol.
Extra Details:: Recombinant full-length human CDK2 a.a.1-298 (end), MW = 34kD with no tag, and CyclinA2 a.a.174-432 (end) with N-terminal His-GST tags, MW=57kD. Both were co-expressed in a baculovirus Sf9 insect cell expression system. The CDK2 gene accession number is NM_001798 and the CyclinA2 gene accession number is NM_001237. Recombinant protein corresponding to active human CDK2, expressed in baculovirus Sf9 insect cell expression system. Molecular Weight: ~34kD, 57kD Specific Activity: 203pmol/min/ug Assay Conditions: Assay was done in a Kinase buffer using Histone H1 (Cell Signaling; 0.1mg/ml at final concentration) as a substrate with 20uM ATP at 30oC for 45 min. Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.