Caspase-6, Active, Recombinant, Human (CASP6, Caspase 6)

Size

£873.00

SKU:: 171841
Molecular Weight:: 201811
Purity:: ~93%
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2mM KCl, 320mM imidazole, 0.01% Tween 20, 20% glycerol.
Extra Details:: Human Caspase 6 (CASP6), GenBank Accession No. NM_001226), full length, amino-acids 1-293 (end), with C-terminal His-tag, expressed in an E. coli cell expression system. Pro-caspase-6 is activated by autocleavage to yield 20kD and 18kD proteins (large subunit) and an 11kD small subunit (His-tag) that dimerize to form the active enzyme. Source: Recombinant protein corresponding to aa1-293 of Caspase-6 at the C-terminus, fused to His-tag expressed in E.coli. Molecular Weight: Large Subunit: ~20kD, ~18kD Small Subunit: ~11kD Specific Activity: 1720pmol/min/ug Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap