BirA, Recombinant (Sf9, Ligase BirA)

Size

£1,117.00

SKU:: 171833
Molecular Weight:: 37
Purity:: ~90%
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2mM KCl, 0.04% Tween 20, 80mM imidazole, 20% glycerol.
Extra Details:: Biotin ligase BirA, GenBank Accession No. AP012306, a.a.2-321 (end) with N-terminal His-FLAG tag, MW=37kD, expressed in a baculovirus infected Sf9 cell expression system. Source: Recombinant protein corresponding to aa2-321from BirA at N-terminus, expressed in a baculovirus infected Sf9 cell expression system. Molecular Weight: ~37kD AA Sequence: MHHHHHHDYKDDDDKKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTL RDWGVDVFTVPGKGYSLPEPIQLLNAKQILGQLDGGSVAVLPVIDSTNQYLLDRIGEL KSGDACIAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMA EVLRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVE ESVVNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFIN RPVKLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.