BirA, Recombinant (Biotin ligase)

Size

£1,117.00

SKU:: 171832
Molecular Weight:: 37.1
Purity:: ~65%
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2mM KCl, 20% glycerol.
Extra Details:: Recombinant protein corresponding to aa2-321 of BirA, with a N-Terminal His-FLAG tag expressed in E. coli (NP_418404). Molecular Weight: ~37.1kD Enzyme Activity: BirA (10ug/ml) completely biotinylated 10uM of substrate MBP-Avi at 30°C for 30 minutes in the standard reaction buffer containing 50mM Bicine pH 8.3, 10mM ATP, 10mM MgOAc, 50uM d-Biotin Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap