ALK, Recombinant, Human (Anaplastic lymphoma receptor tyrosine kinase)

Size

£912.00

SKU:: 171821
Molecular Weight:: 89
Purity:: ~60%
Storage Conditions:: -70°C
Supplier:: United States Biological
Format:: Supplied as a liquid in 40mM Tris-HCl, pH 8.0, 110mM NaCl, 2.2mM KCl, 3mM DTT, 20% glycerol.
Extra Details:: Human anaplastic lymphoma receptor tyrosine kinase (ALK), also known as CD246 and NBLST3, (GenBank Accession No. NM_004304), a.a. 1058-1620 (end) with N-terminal GST tag, MW = 89kD, expressed in a Baculovirus infected Sf9 cell expression system. Source: Recombinant protein corresponding to aa1058-1620 from human ALK at N-terminal, fused to GST tag, expressed in baculovirus infected Sf9 cell expression system. Molecular Weight: ~89kD Specific Activity: 77 pmole/min/ug Assay Conditions: Assay was done in a Kinase buffer using 0.2 mg/ml IGF1Rtide as a substrate with 20 uM ATP at 30oC for 40 min. The amount of ATP transferred was calculated using Kinase-Glo reagent (Promega). Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.