171733
Prostate Specific Antigen (PSA, Total) (HRP)
Size
£1,081.00
- SKU:
- 171733
- Purity:
- Purified by S-300 Column Chromatography from ascites.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human PSA.
- Clone:
- 14D162
- Format:
- Supplied as a liquid in PBS, pH 7.2, containing BSA and Proclin. Labeled with Horseradish Peroxidase (HRP).
- Extra Details:
- PSA is a chymotrypsin-like serine protease (kallikrein family) produced by the prostate epithelium, and abundant in seminal fluid. PSA can be detected in the sera of patients with prostatic carcinoma. It is predominantly complexed to a liver-derived serine protease inhibitor, alpha-1-antichymotrypsin (ACT). A higher proportion of serum PSA is complexed to ACT in prostate cancer than in benign prostate hyperplasia. Anti-PSA is useful in the positive identification of metastatic deposits of prostatic origin. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 1:1000 Optimal dilutions to be determined by the researcher. Affinity Constant: 6x10e9 L/mol (purified unconjugate form). Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice