Microglobulin, Beta 2

Size

£492.00

SKU:: 171665
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified human Microglobulin, Beta 2
Clone:: 14D121 (B2M-01)
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Extra Details:: Applications: Suitable for use in ELISA, RIA, Immunohistochemistry, Flow Cytometry, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 2-4ug/ml; positive control: U937 human histiocytic lymphoma cell line, RAJI human Burkitt lymphoma cell line, negative control: EL4 mouse lymphoblastic lymphoma cell line; both reducing and non-reducing conditions. Immunohistochemistry (paraffin sections): 10ug/ml; positive tissue: kidney glomeruli. Flow Cytometry: 0.5-4ug/ml; positive control: peripheral blood lymphocytes, negative control: DAUDI human Burkitt lymphoma cell line, erythrocytes. RIA: The dissociation constant of the antibody soluble beta2-microglobulin is 1.5x10-8 mol/l as determined by competitive RIA. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.