CD63 (CD63 Activated Platelets) (FITC)

Size

£1,023.00

SKU:: 171511
Purity:: Purified from ascites
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human cytochrome B enriched cells.
Clone:: 14D43
Format:: Supplied as a liquid in PBS, pH 7.2, 2mg/ml BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Extra Details:: CD63 is a multi-pass, lysosomal membrane protein belonging to the tetraspan (or tetraspanin) transmembrane protein family. The structure of CD63 is comprised of 4 domains spanning the membrane; two unequally sized extracellular loops and two, short domains within the cytoplasm, which are thought to participate in signal transduction for some cell types. CD63 also contains a characteristic lysosomal targeting motif, GYWVM, within its C-terminal sequence. Although the exact function of CD63 has yet to be elucidated, it has been implicated as having a role in growth regulation and to be involved in the initial stages of melanoma tumor progression. Expression of CD63 has been observed in dysplastic nevi, hematopoietic cells, radial growth phase primary, and in tissue macrophages. Studies have suggested a role for CD63 in macrophage infection from CCR5-mediated HIV-1. Loss of CD63 expression has been observed within in a patie4nt suffering from a rare, genetic disorder known as Hermansky-Pudlak syndrome (HPS). Applications: Suitable for use in Immunofluorescence and Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 20ul/10e6 platelets or 20ul/5x10e5 cells/test. Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 AG1.4 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice