171499
CD28 (CD28 Antigen, CD28 Molecule, T cell Antigen CD28, Tp44, T cell Specific Surface Glycoprotein, T cell Specific Surface Glycoprotein CD28)
Size
£512.00
- SKU:
- 171499
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- DC28.1.3.3 murine T cell hybridoma transfected with human CD28 cDNA
- Clone:
- CD28.2
- Format:
- Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide
- Extra Details:
- CD28 is a T-cell surface homodimeric 44kD molecule that belongs to the Ig superfamily (1). CD28 is involved in cell adhesion T-B through the binding to its ligand B7/BB1, which is also the ligand for CTLA- 4. CD28-B7 interaction is an important cosignal that allows T-cell proliferation and lymphokine production. CD28 is associated to a PI3 kinase activity (2). The majority of CD4+ T-cells and 50% of CD8+ T-cells express CD28 (3). The monoclonal antibody induces T-cell proliferation in costimulation with CD2 monoclonal antibodies (2). It also inhibits the CD4+ proliferation in the allogeneic T-cell response (2). This antibody has been studied at the Vth International Workshop on Human Leucocyte Differentiation Antigens in Boston (1993). Applications: Suitable for use in Flow Cytometry, Immunoprecipitation, Immunocytochemistry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1-12ug/ml Immunohistochemistry: frozen sections Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice