170933
MAP3K8 (TPL2, C-COT, Cancer Osaka thyroid oncogene, COT, Ewing Sarcoma Transformant, EST, MEKK8, Proto-oncogene c-Cot, Tumor Progression Locus 2, Protein Kinase Cot, Tpl-2, ESTF)
Size
£709.00
- SKU:
- 170933
- Application:
- IHC-Fr
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to 19aa from the N-terminus of human MAP3K8/TPL2. Species sequence homology: hamster, bat and canine.
- Format:
- Supplied as a liquid in PBS, 0.09% sodium azide.
- Extra Details:
- MEKK8, known as COT for cancer Osaka thyroid, is a MAP3K type protein kinase that was identified by its oncogenic transforming activity in cells. COT activates the JNK cascade by phosphorylating MKK4/MAP2K4 and the ERK cascade by phosphorylating MEK1/MAP2K1. COT also has been implicated in the induction of NF-kappaB-dependent transcription and the production of TNF-alpha and interleukin-2 during T lymphocyte activation. At least two protein isoforms which result from the use of alternative translation initiation sites and differ in their N-terminus have been reported. In the COT oncoprotein, the C-terminal 70aa of normal COT are replaced by 18 novel residues; however, studies have shown that the COT gene can be activated as an oncogene by overexpression as well as by amino acid rearrangements. COT has been shown to contribute to Epstein-Barr virus-encoded latent infection membrane protein 1-induced NF-kappaB signaling downstream of TRAF2. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 15ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice