Granzyme A, Recombinant, Human, His-Tag

Size

£812.00

SKU:: 169686
Molecular Weight:: 28
Purity:: ≥95% (SDS-PAGE, SEC-HPLC)
Storage Conditions:: -20°C
Supplier:: United States Biological
Format:: Supplied as a lyophilized powder from 25mM MES, 300mM sodium chloride, 10mM calcium chloride, 5% trehalose. Reconstitute with sterile 25mM HEPES, 150mM sodium chloride, 10mM calcium chloride pH 7.5.
Extra Details:: Granzyme A is a member of the granzyme family. Granzyme A is the most abundant serine protease in cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Granzyme A has a specific function in CTL and NK cells. It induces caspase-independent cell death when introduced into target cells by perforin. Human granzyme A is synthesized as a precursor (262 residues) with a signal peptide (residues 1-26), a propeptide (residues 27-28) and a mature chain (residues 29-262). Recombinant protein corresponding to Cys26-Val262 of human Granzyme A with a C-terminal 10 His tag expressed in NSO cell line. Swiss/UniProt Accession: P12544. Synonyms: GZMA; Granzyme-1; CTL Tryptase; Cytotoxic T-lymphocyte-associated Serine Esterase 3; CTLA3; Cytotoxic T-lymphocyte Proteinase 1; Fragmentin-1; Hanukkah Factor; H Factor; HF; HFSP Structure: Pro form Molecular Weight: 252 amino acid recombinant protein has a predicted molecular mass of approximately 28kD. The protein migrates at about 35kD in DTT-reducing conditions and about 55kD in non-reducing conditions by SDS-PAGE.The predicted N-terminal amino acid is Cys. Activity: Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Gly-Arg-ThioBenzyl ester (Z-GR-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468. The specific activity is >5,000 pmol/min/g, as measured under the described conditions. Endotoxin: ≤1.0 EU per 1ug of the protein by the LAL method. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute at 100ug/ml in sterile 25 mM HEPES, 150mM NaCl and 10mM CaCl2, pH 7.5. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice