TFIIEb (Transcription Factor IIEb Subunit p34)

Size

£603.00

SKU:: 169583
Application:: WB
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to full-length p34 subunit of TFIIEb.
Format:: Supplied as a liquid in PBS.
Extra Details:: Human Transcription Factor IIE (TFIIE) is composed of 56kD (alpha) and 34kD (beta) subunits and is shown to be a heterotetramer. The 56kD subunit contains a region similar to a zinc-binding domain and a region sharing homology with the catalytic loop of a kinase domain. TFIIE binds to RNA polymerase II in solution and joins the preinitiation complex probably concomitant with RNA polymerase II and TFIIF. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:200-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.