SP1 (Sp1, Transcription Factor Sp1) (Not for Export EU)

Size

£603.00

SKU:: 169580
Application:: WB
Purity:: Serum.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Sp1 generated from baculovirus system.
Format:: Supplied as a liquid in PBS.
Extra Details:: Sp1 was the first transcription factor to be cloned and characterized. Analysis of structure and function has re- vealed that Sp1 can be separated into discrete functional domains. The DNA-binding domain consists of three zinc fingers that specifically bind to the GC-box element. Sp1 contains at least four separate transcriptional activation domains. Two of these domains are glutamine-rich, a well- characterized motif found in several other transcription factors. In addition to transcription, Sp1 has been linked to cell growth, cancer, Huntington disease and other disor- ders through transcriptional regulation or specific protein-protein interactions. The function of Sp1 can be regulated by phosphorylation and glycosylation. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.