Interleukin 4 (IL-4)

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£513.00

SKU:: 169546
Purity:: Purified.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human IL-4.
Clone:: 13F222
Format:: Supplied as a lyophilized powder in PBS. Reconstitute with 500ul sterile ddH2O.
Extra Details:: IL-4 is a pleiotropic cytokine produced by activated T-cells. IL-4 is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL-13, which may contribute to many overlapping functions of this cytokine and IL-13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL-3, IL-5, IL-13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL-13. IL-4, IL-13 and IL-5 are found to be regulated coordinately by several long-range regulatory elements in an over 120kb range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. Applications: Suitable for use in Direct ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilution: Direct ELISA: 1:10,000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice