EGF (Epidermal Growth Factor)

Size

£685.00

SKU:: 169527
Purity:: Purified.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human EGF.
Clone:: 13F207
Format:: Supplied as a lyophilized powder in PBS. Reconstitute with 500ul sterile ddH2O.
Extra Details:: Epidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide. EGF stimulates the growth of various epidermal and ep- ithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA (direct): 1:10,000 Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.