Vimentin (FITC)

Size

£704.00

SKU:: 169221
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Vimentin extract of bovine lens
Clone:: RV202
Format:: Supplied as a liquid in PBS, 0,1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Extra Details:: Vimentin (57kD) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:100-1:200 Western Blot: 1:100-1:1000 Immunohistochemistry (frozen): 1:100-1:200 With avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. Optimal dilutions to be determined by the researcher. Hybridoma: SP2/0-Ag14 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.

Shipping Conditions:: Blue Ice