VCAM-1 (Vascular Cell Adhesion Molecule-1)

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£659.00

SKU:: 169216
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Fused with the NS-1 nonsecretory murine myeloma line.
Clone:: 4B9
Format:: Supplied as a liquid in PBS, 0.09% sodium azide.
Extra Details:: Vascular cell adhesion molecule-1 (VCAM-1) is induced on human umbilical vein endothelium (HUVE) by recombinant human tumor necrosis factor - a (rh TNF-a), rh interleukin-1 (IL-1) and lipopolysaccharide (LPS) and is involved in adherence of lymphocytic cell lines to HUVE. VCAM-1 is only minimally expressed on unstimulated endothelium. Molecular cloning and sequencing of VCAM-1 predicts a 69kD core protein with 6 potential N-linked glycosylation sites, if the protein is fully glycosylated a mature protein of about 90kD is yielded. VCAM-1 mediates a component of the adherence of Peripheral Blood Lymphocytes (PBL) to rh TNF-stimulated HUVE, this CD18 independent mechanism of lymphocyte adherence to cytokine stimulated endothelium may be an important pathway of lymphocyte emigration at sites of inflammation and immune reaction. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:25-1:200 Western Blot: 1:100-1:1000 Immunohistochemistry (frozen): 1:25-1:200. With avidin-biotinylated horseradish peroxidase complex (ABC) as detection reagent. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice